Micropropagation of Strawberry Plants (Fragaria x ananassa Duch.)

Strawberry is a plant that is quite popular in Indonesia and the world. From year to year, the demand for strawberries in Indonesia continues to increase. This resulted in the expansion of cultivation and increasing demand for strawberry seedlings.

Until now the strawberry seedlings in Indonesia are still using the stolon. Seeds obtained from the stolon has several limitations, among other seeds could not be available in large quantities and uniform, a decrease in the quality of seed when parent plants have been taken stolonnya taken repeatedly, and allows the infected pathogens from parent plants.

In 1952, Morel and Martin was able to create disease-free strawberry seed through meristem culture. Since applied to strawberries, (Adams 1972, Mullin et al. 1974, Nishi et al. 1973) meristem culture taken from the stolon applied commercially to eliminate pathogens on strawberry seedlings (Boxus 1974, Boxus et al. 1977). Zebrowska et al. (2003) mensitasi some literature that shows that plants produced by micropropagation system has more stolon, tiller number increased and fruit production also increased.

Some of the composition of media for micropropagation of strawberries has been published, mostly using MS medium (Murashige and Skoog 1962) supplemented with the type and concentration of growth regulator that is different. One study has shown that the concentration of the optimal growth regulator can vary between cultivars of each other (Cerovic and Ruzic 1989). Table 1 presents examples of the composition of media for micropropagation of strawberry.
Tabel 1. Media mikropropagasi stroberi yang direkomendasikan Boxus (1974)

Component Initiation media Media Multiplication Rooting media
Makronutrien Knop (1865) Knop (1865)

Knop (1865)

Mikronutrien Murashige and Skoog (1962) Murashige and Skoog (1962) Murashige and Skoog (1962)
Vitamin Murashige and Skoog (1962) Murashige and Skoog (1962) Murashige and Skoog (1962)
ZPT (mg/L)

BA 0.1

IBA 1.0

GA3 0.1

BA 1.0

IBA 1.0

GA3 0.1
IBA 1.0
Sugar (g/L) Glukosa 40 Glukosa 40 Glukosa 40
Jell (g/L) 8.0
8.0 8.0

Micropropagation method using meristem culture begins with the isolation of stolon meristem taken from the parent plant and then planted on initiation media (Table 1). Meristem grown on this medium to form plantlets. Plantlets that formed later transferred to multiplication media. In this media, new shoots will be formed quickly. Shoots that have been mature enough then transferred to rooting media. Plants that have rooted and are ready to acclimatized will be formed after 6-8 weeks.

 

 


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